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Plates were placed in a motorized stage microscope (EVOS Auto FL 2, Thermo Fisher Scientific, Waltham, MA) equipped with an on-stage incubator and maintained at 5% CO 2 and 37C

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Characterisation of reduced oocyte quality Impaired maturation of oocyte nuclei Increased aneuploidy associated with meiotic segregation errors is a significant factor contributing to the reduced fertility of germ cell senescence, characterised by incorrect chromosome numbers, which may be due to chromosome segregation errors during meiosis [18]

uric acid accounts for more than 50% of all antioxidant activity in the human serum

Traditional Microneedling Traditional microneedling mainly stimulates collagen through controlled micro-injuries, while RF microneedling stimulates collagen and tightens the skin by combining microneedling with radiofrequency energy